Slice
Electrophysiology
Slice electrophysiology can be used to identify specific drug targets, evaluate efficacy, investigate mechanism of action and thus aid assay development to translate such findings into high and medium throughput screens.
Slice electrophysiology at NeuroSolutions Ltd. includes:
- Blind or infra-red visualised patch clamp recordings from brain and spinal cord slices
- Analysis of drug action on excitatory and inhibitory pre- and post-synaptic activity, both fast (ligand gated ion channel-mediated) and slow (e.g. GPCR-mediated)
- Direct measurement of drug action on cell excitability
- Extracellular recordings, including measurement of long-term potentiation and long-term depression in, for example, hippocampal slices using field potential recordings
- Multi-electrode array (MEA) recording 128 channels simultaneously
NeuroSolutions' slice experts can provide a broad range of pharmacological and physiological data using the following techniques:
- Current/voltage-clamp recordings
- Perforated patch recordings
- Pre-/post-synaptic activity
- Paired recordings
- Field potential recordings
- Quantitative/single-cell RT-PCR
